| Step 1. Load an Image |
 |
|
SearchLight Array Analyst Software can analyze protein array images from a variety of instruments which save an image as a 16-bit tiff. |
Step 2. Define the Array |
 |
|
Indicate the number of rows and columns on the plate or slide. Indicate the number of rows and columns within the array. Select an imaging system. |
Step 3. Define the Analytes
Indicate the proteins being measured in the array. Once analytes are selected, starting concentrations will be entered automatically, based on the current SearchLight concentration file.
|
 |
Step 4. Define the Plate Layout |
 |
|
Indicate wells containing standards, samples, and controls. Sample names can be imported from Excel or entered manually. Once complete, an analysis protocol can be saved for future experiments. |
Step 5. Align the Image |
 |
|
Many images can be aligned using the Auto Alignment feature. Manual alignment techniques may also be applied to the entire image or small sections of an image. |
Step 6. Calculate Results
The software automatically plots a standard curve for each analyte and calculates mean, %CV (coefficient of variation), standard deviation, and the Linear Quality for each unknown sample for each analyte.
|
 |
Step 7. Export to Excel
The Excel report plots analyzed data by analyte for simple review of results. Standard backfits, LOD, and LOQ are included for each standard curve. |
 |
A final summary table shows results for all analytes for each unknown sample. |
 |
